田 珍 劉小玲 丁益群 劉旭博

摘要采用皮質(zhì)醇混飼投喂的方法,模擬自然條件下的慢性應(yīng)激,研究皮質(zhì)醇對(duì)黃顙魚成魚吞噬細(xì)胞呼吸暴發(fā)和血清補(bǔ)體旁路溶血活性(ACH50)的影響。研究結(jié)果與結(jié)論如下。皮質(zhì)醇混飼投喂可使血清皮質(zhì)醇濃度持續(xù)顯著升高,各周對(duì)照組血清皮質(zhì)醇含量均無(wú)顯著性差異,證明采用皮質(zhì)醇混飼投喂的方法可模擬自然慢性應(yīng)激情況下的血清皮質(zhì)醇濃度持續(xù)顯著升高。從第2周開始頭腎吞噬細(xì)胞的呼吸暴發(fā)功能顯著下降(P<0.05),證明皮質(zhì)醇對(duì)頭腎吞噬細(xì)胞的殺菌能力產(chǎn)生顯著抑制作用。10mg/kg劑量組,ACH50在投喂第2、第5、第6周顯著低于對(duì)照組(P<0.05),100mg/kg劑量組,ACH50從第2周開始一直都極顯著低于同期對(duì)照組(P<0.01),證明皮質(zhì)醇顯著抑制血清補(bǔ)體旁路溶血活性,且血清皮質(zhì)醇濃度越高其抑制作用越強(qiáng)。

關(guān)鍵詞皮質(zhì)醇;黃顙魚;頭腎吞噬細(xì)胞;呼吸暴發(fā);血清補(bǔ)體旁路活性

中圖分類號(hào) Q571 文獻(xiàn)標(biāo)識(shí)碼A文章編號(hào) 1007-5739(2009)09-0232-02

EffectsCortisolontheActivityofPhagocytesandComplementinAdultPelteobagrusfulvidraco

TIAN ZhenLIU Xiao-ling *DING Yi-qunLIU Xu-bo

(College of Fisheries, Huazhong Agricultural University, Wuhan Hubei430070)

AbstractSimulating natural chronic stress,Method of incorporation of cortisol in diet was used to study the effects of cortisol on the phagocyte respiratory burst and the serum alternative complement hemolytic activity(ACH50) of adult yellow catfish(Pelteobagrus fulvidraco). The results and conclusions are as follows.The cortisol incorporated in the diet resulted in sustained elevation of serum cortisol concentration, while there was no statistic difference among control groups. This result indicated that the method of incorporation of cortisol in the diet can be used to imitate natural stress response,which was usually characterized with a continuous increase of the serum cortisol concentration.From the second week,the respiratory burst of the head kidney phagocytes significantly decreased(P<0.05). This result indicated that cortisol significantly inhibited the bactericidal ability of the head kidney phagocytes.ACH50 in the 10 mg/kg group decreased significantly(P<0.05)in the second、fifth and sixth weeks,and decreased highly significantly(P<0.01)in the 100 mg/kg group from the second week to the sixth week. This result indicated that cortisol significantly suppressed the ACH50,and the higher the serum cortisol levels the more pronounced the suppression.

Key wordscortisol;Pelteobagrus fulvidraco;head kidney phagocyte;respiratory burst;serum alternative complement hemolytic activity