微生物學(xué)
封面介紹:Phylogenetic trees of Epichlo? spp. and Neotyphodium spp. based on maximum parsimony(MP)analysis of sequences from intron portions of act genes. The tree is 323 steps in length,consistency index = 0.8421,retention index = 0.9706. Maximum parsimony(MP)trees were constructed in PAUP 4.0b10 package. MP trees implemented using the heuristic search based on tree bisection-reconnection(TBR)branch swapping and 100 random addition replicates. Alignment gaps were treated as missing information. Nucleotide substitutions were unordered and unweighted. Bootstrap support values were calculated by 1000 replications. Branches with bootstrap values > 50% are shown.
基于act序列中國西部披堿草屬植物所帶內(nèi)生真菌的系統(tǒng)演化
宋輝,李秀璋,鮑根生,等
目的:研究中國西部披堿草屬植物(Elymus spp.)所帶無性世代內(nèi)生真菌的系統(tǒng)進(jìn)化關(guān)系.方法:克隆 15條分離于中國西部披堿草屬植物無性世代內(nèi)生真菌肌動蛋白基因(act)序列,并與下載自 GenBank中內(nèi)生真菌的 act序列共同構(gòu)建系統(tǒng)進(jìn)化樹和網(wǎng)絡(luò)結(jié)構(gòu)拓?fù)鋱D.結(jié)果:供試無性世代內(nèi)生真菌act序列均為單拷貝基因.系統(tǒng)進(jìn)化關(guān)系表明,它們與分離于北美洲披堿草屬植物的有性世代內(nèi)生真菌 Epichlo? elymi存在不同的起源,而與分離于北美洲直芒草(Achnatherum eminens)的無性世代內(nèi)生真菌 Neotyphodium chisosum具有較近的親緣關(guān)系.進(jìn)一步研究發(fā)現(xiàn),由北美洲直芒草無性世代內(nèi)生真菌N. chisosum形成的單元型Hap 8與本研究菌株所組成的單元型Hap 3之間可能存在基因流.
內(nèi)生真菌;披堿草屬植物;肌動蛋白;系統(tǒng)發(fā)育
來源出版物:微生物學(xué)報(bào),2015,55(3): 273-281聯(lián)系郵箱:南志標(biāo),zhibiao@lzu.edu.cn