Lin CHEN,You ZHOU,2,Weizhuo PENG,Xiu LIU,2,Caiping HUANG,Chenzhong JIN,2*,Jiajia LIU,Jingbo LI,2,Yanan DENG,Meng QIN

1.College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Loudi 417000,China;

2.Key Laboratory of Pesticide Harmless Application of Hunan Higher Education,Hunan University of Humanities,Science and Technology,Loudi 417000,China

The Control Effect of Prevention and Control Technology Combination against Southern Rice Black-Streaked Dwarf Disease

Lin CHEN1,You ZHOU1,2,Weizhuo PENG1,Xiu LIU1,2,Caiping HUANG1,Chenzhong JIN1,2*,Jiajia LIU1,Jingbo LI1,2,Yanan DENG1,Meng QIN1

1.College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Loudi 417000,China;

2.Key Laboratory of Pesticide Harmless Application of Hunan Higher Education,Hunan University of Humanities,Science and Technology,Loudi 417000,China

[Objective]This study aimed to explore a better prevention and control technology combination against southern rice black-streaked dwarf disease.[Method] The control effects of seed dressing,antiviral agents and fly net along with 25% pymetrozine against southern rice black streaked dwarf disease were determined. [Result]When the seeds were dressed with 60%imidacloprid(FS,2 g a.i./kg),and the rice seedlings were sprayed once with 25%pymetrozine(WP,360 g/hm2)10 d before the transplanting and sprayed twice with 30%Dufulin(WP,800×)at threeleaf stage and 7 d after the transplanting respectively,the control efficiency reached 88.05%.When fly net and pesticides were applied simultaneously,the fly net was lifted and 25%pymetrozine(WP)was spayed once on the rice seedlings at six-leaf stage and the seedlings were hardened for 3 d and sprayed once with 25% pymetrozine(WP)10 d after the transplanting,the control efficiency reached 80.50%.[Conclusion]Seed dressing or applying antiviral agents alone can not better control diseases.The southern rice black-streaked dwarf disease can be better controlled by seed dressing,along with spraying of planthopper-killing agents at two-leaf stage,3 d before the transplanting and 7 d after the transplanting,respectively.If condition allow,fly net can be installed to achieve better control effect.

Southern rice black-streaked dwarf disease;Planthopper;Agents;Seed dressing;Fly net

S outhern rice black-streaked dwarf virus,first discovered, identified and named by Chinese scholars,is a new virus species endangering crops,and it belongs to Fijivirus,Reoviridae[1].The incidence and prevalence of southern rice blackstreaked dwarf disease is mediated by Sogatella furcifera,a kind of migratory pest in the major rice-producing areas in China[2].In recent years,the incidence area of southern rice blackstreaked dwarf disease in the major rice-producing areas in southern China has been expanded rapidly,and the degree of harm has also been increased significantly.Especially since 2009,the southern rice black-streaked dwarf disease has caused large-area harvest failure,resulting in huge losses in agricultural production.The southern rice black-streaked dwarf dis ease is a huge potential threat to rice production safety in China[3-4].The white backed planthoppers move to Hunan province since early April every year.Their randomized landing and uncertain infection rate have brought great difficulties to timely prevention and control[5].It indicates that the inci-

In order to explore drug application and prevention and control techniques with better control effect against southern rice black-streaked dwarf disease in central Hunan Province,the field tests of seed dressing along with antiviral agents and fly net along with pesticides were carried out in 2014 so as to provide technical support for prevention and control of southern rice black-streaked dwarf disease.

Materials and Methods

Materials and agents

The tested rice cultivar was Huailiangyou 608.A total of two kinds, 30-mesh and 40-mesh,of fly net were adopted in this study.The tested pesticides were shown in Table 1.

Control effect of seed dressing along with antiviral agents against southern rice black-streaked dwarf disease

Test treatmentsAt the three-leaf stage,the rice seedlings were sprayed once;the rice seedlings were sprayed once 7 d after the transplanting.The sprayed drugs were shown in Table 2.

Drug application methodsAfter rice seeds started to germinate,60% imidacloprid FS was mixed with dry seeds according to the amount of 2 g a.i./kg.The imidacloprid was first dissolved in clean water(100 ml),and the solution was then mixed with germinated rice seeds.The sowing was performed 3 d after the seeds were dried. There were three replicates for each treatment.A total of 39 plots were designed.In the very early stage, the area of each nursery was 30 m2. The area of field corresponding to each nursery was 120 m2.

Control effect of fly net along with pesticides against southern rice black-streaked dwarf disease

Test treatmentsThe test treatments were shown in Table 3.

Drug application methodFor each treatment,the 25%pymetrozine was first diluted with water(m/m,6/1 000) and then sprayed on rice seedlings.Survey method

At late tillering stage and yellow ripening stage,the incidences of southern rice black-streaked dwarf disease were surveyed.The five-point sampling method was adopted.A total of 100 seedling plants were selected from each plot.The infected plant number of each plot was recorded, and then the infected plant rate and control efficiency were calculated.

The survey standards were as follows:at late tillering stage,the rice plants were dwarf and stiff,and leaf color was dark green;at yellow ripening stage,the nodules during the bottom first and third stem nodes of rice plants were very rough.

Calculation method

Infected plant rate(%)=Infected plant number/Surveyed plant number× 100%;

Control efficiency(%)=(Infectedplant rate in the control group-Infected plant rate in treatment group)/Infected plant rate in the control group×100%.

Table 1Selected pesticides for test

Table 2Treatments of seed dressing and antiviral agents

Table 3Treatments of fly net and pesticides

Results and Analysis

Control effect of seed dressing along with antiviral agents against southern rice black-streaked dwarf disease

As shown in Table 4,the antiviral agents were sprayed alone in treatments 1,2,3 and 4,but the control effect were all not ideal.At the milk ripen stage,the control efficiencies of Dufulin,ultra-sensitive protein,lentinan and Ningnan mycin were 50.99%, 51.73%,42.67%and 47.99%,respectively.There were no significant differences in control efficiency among the four treatments.In treatments 5,6,7 and 8,the antiviral agents were sprayed along with 60%imidacloprid seed dressing.The control efficiencies of treatments 5,6,7 and 8 were significantly higher than those of treatments 1,2,3 and 4 at the milk ripen stage. Among them,the control efficiency of treatment 5[dressing seeds with 60% imidacloprid FS(2 g a.i./kg);spaying 30%Dufulin WP(800×)twice at the three-leaf stage and 7 d after the transplanting]was highest(80.66%). The treatments 6,7 and 8 also showed better control effect.In treatments 9, 10,11 and 12,seed dressing,spraying antiviral agents and spaying pesticides against planthoppers were combined. Better control effect was obtained,and the control efficiencies of the four treatments were all more than 85%.

Control effect of fly net along with pesticides against southern rice black-streaked dwarf disease

Table 5 showed that the control effect of 40-mesh fly net against southern rice black-streaked dwarf disease was better than that of 30-mesh fly net. Under conditions of the same agents and amounts,the control efficiencies of 40-mesh fly net were increased by 2.50%,3.75%,12.00%,15.51%, 0.12%and 1.20%respectively compared with those of 30-mesh fly net. The treatment 12(lifting fly net at sixleaf stage;spaying 25%pymetrozine WP once;hardening seedlings for 3 d before transplanting;spaying 25% pymetrozine WP once 10 d after transplanting)showed the highest control efficiency(80.50%).At the condition of the same-mesh fly net,the control effect of fly net along with planthopper-killing agents was better than that of fly net alone.

Table 4Control effect of seed dressing and antiviral agents against southern rice blackstreaked dwarf disease%

Table 5Control effect of fly net and pesticides against southern rice black-streaked dwarf disease%

Conclusions and Discussion

The seed dressing,planthopper control and antiviral agents alone all showed unideal control effect against southern rice black-streaked dwarf disease.In the treatment of seed dressing with 60%imidacloprid FS and spraying of 25%pymetrozine at twoleaf stage,3 d before transplanting and 7 d after transplanting,the control efficiency was 62.07%;if 30%Dufulin WP(800×)was sprayed additionally at three-leaf stage,the control efficiency was increased to 68.85%;Furthermore,if 30%Dufulin WP(800×)was sprayed additionally 7 d after the transplanting,the control efficiency was increased to 78.49%.When the three kinds of control technology were combined,the control effect was more ideal,i.e.,for the treatment of seed dressing with 60%imidacloprid FS, spraying of 25%pymetrozine at twoleaf stage,3 d before transplanting and 7 d after transplanting and spraying of 30%Dufulin WP(800×)at threeleaf stage and 7 d after the transplanting,the control efficiency was more than 85%.

Based on study results above,it could be concluded that antiviral agents and planthopper-killing agents should be used combinedly due to difficult prevention and control of southern rice black-streaked dwarf disease[8].In order to achieve a better prevention and control effect in production,the southern rice blackstreaked dwarf virus should be controlled on one hand,and on the other hand,the spreading of planthoppers must be cut off[9-10].

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Responsible proofreader:Xiaoyan WU

組合防控技術(shù)對南方水稻黑條矮縮病的防治效果研究

陳琳1，周友1，2，彭偉卓1，劉秀1，2，黃彩萍1，金晨鐘1，2*，劉佳佳1，李靜波1，2，鄧亞男1，覃夢1
（1.湖南人文科技學(xué)院農(nóng)業(yè)與生物技術(shù)學(xué)院，湖南婁底417000；2.農(nóng)藥無害化應(yīng)用湖南省高校重點實驗室，湖南婁底417000）

[目的]探究對南方水稻黑條矮縮病防治效果較好的防控技術(shù)。[方法]采用田間試驗方法測定了藥劑拌種和抗病毒劑以及防蟲網(wǎng)+25%吡蚜酮對南方水稻黑條矮縮病的防效。[結(jié)果]用60%吡蟲啉FS 2 g a.i./kg干種子量拌種、移栽前10 d 25%吡蚜酮WP 360 g/hm2噴1次，30%毒氟磷WP 800倍在水稻秧苗3葉1心期施藥1次、移栽后7 d再施藥1次，防效達到88.05%；用防蟲網(wǎng)+殺蟲劑在6葉1心期揭網(wǎng)，噴施25%吡蚜酮WP 1次，煉苗3 d移栽10 d后，再次噴施25%吡蚜酮WP 1次，所得防效為80.50%。[結(jié)論]只用拌種或施抗病毒劑的方法不能很好的控制病害的為害。藥劑拌種結(jié)合在秧苗2葉1心期、移栽前3 d的及移栽后7 d各噴一次殺飛虱藥劑防效較好，有條件的地方兼用防蟲網(wǎng)效果更好。

南方水稻黑條矮縮??；飛虱；藥劑；拌種；防蟲網(wǎng)dence of southern rice black-streaked dwarf disease in southern China is closely related to immigration amount, immigration time and infection rate of white backed planthoppers.A series of prevention measures should be adopted to prevent and control southern rice black-streaked dwarf disease in rice.First,the white backed planthoppers must be better controlled at the seedling stage[2,6].Moreover,some long-acting drugs are also need to be used in the long-term control of southern rice black-streaked dwarf planthoppers,thus the harms of southern rice black-streaked dwarf disease to rice will be reduced[7].

湖南省教育廳科技計劃項目（14C0606）；湖南人文科技學(xué)院青年基金項目（2012QN10）；湖南人文科技學(xué)院產(chǎn)學(xué)研合作引導(dǎo)基金（2013CXY04）；湖南省大學(xué)生研究性學(xué)習(xí)和創(chuàng)新性實驗計劃項目（2013-389）。

陳琳（1992-），女，湖南株洲人，本科，專業(yè)：農(nóng)學(xué)，主要研究方向為農(nóng)作物有害生物防控，E-mail：532479626@qq.com。*通訊作者，E-mail：hnldjcz@sina.com。

2015-03-18

修回日期 2015-06-03

Supported by Science and Technology Plan Project of Department of Education,Hunan Province(14C0606);Youth Foundation of Hunan University of Humanities,Science and Technology(2012QN10);Production-Study-Research Cooperation Guidance Fund of Hunan University of Humanities,Science and Technology(2013CXY04);Research Study and Innovative Experiment Plan Project for College Students in Hunan Province (2013-389).

*Corresponding author.E-mail:hnldjcz@sina.com

Received:March 18,2015 Accepted:June 3,2015