Sun Xuyong(孫煦勇，Organ Transplant Center， No.303 Hosp PLA，Nannning 530021)…∥Chin J Transplant.－ 2011，32(8).－502～505

Apoptosis and oxidative injury of donor islets during isolatio

Sun Xuyong(孫煦勇，Organ Transplant Center， No.303 Hosp PLA，Nannning 530021)…∥Chin J Transplant.－ 2011，32(8).－502～505

ObjectiveTo observe the changes of islet cell apoptosis and oxidation－antioxidation before transplantation，and to explore pathways of islet protection.MethodsFifteen human pancreases were perfused with Hanks solution containing collagenase，then digested and isolated.During the procedure，islet cell apoptosis was detected by TUNEL，SOD and MDA in the pancreas were measured by colorimetric method，and morphologic changes were observed by H －E staining and dithizone staining.ResultsIn the procedure of human islet isolation，especially in the stage of digestion，apoptosis of human islet cells occurred.In the stages of perfusion and digestion，MDA contents reached high levels(6.18±2.38 and 9.21±2.75 nmol/mg protein respectively)，and structures of islets and tissues around islets were damaged.ConclusionIn the stages of perfusion and digestion，apoptosis of islet cells can be caused by oxidation.It suggests that antioxidation is a pathway for protection of islets before transplantation.10 refs，2 figs，1 tab

(Authors)