The Dalian University of Technology (DUT) has disclosed a strain with high yield of 1,3-propanediol coupled with a sequencial inoculation fermentation process. This strain is called Clostridium butyricum DL07, which can produce 104.78 g/L or 94.23 g/L of 1,3-propanediol, respectively, by fermenting pure glycerol or crude glycerol,respectively, to set a highest yield of 1,3-propanediol obtained via fermentation of pure/crude glycerol by using natural micoorganisms reported so far.

At the same time, the sequencial inoculation fermentation process and the automatic substrate fermentation control method also have been proposed to realize a multistage continuous fermentation process, which can make Clostridium butyricum capable of safely producing 1,3-propanediol with high efficiency, high cost-effectiveness, and strong stability to hold out a better commercial application prospect.